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| 05/10/2016 | 16:00 | Dynamo České Budějovice | - | SFC Opava | 2:1 (1:0) | |
| 08/10/2016 | 09:15 | FK Fotbal Třinec | - | FK Baník Sokolov | 2:1 (0:1) | |
| 09:15 | FK Pardubice | - | MFK Frýdek-Místek, a.s. | 0:0 (0:0) | ||
| 15:00 | 1. SK Prostějov | - | FK Ústí nad Labem | 0:1 (0:0) | ||
| 15:00 | MFK Vítkovice | - | Sigma Olomouc | 1:2 (0:0) | ||
| 15:00 | FK MAS Táborsko | - | Baník Ostrava | 2:2 (1:0) | ||
| 09/10/2016 | 09:15 | Viktoria Žižkov | - | 1. SC Znojmo | 1:0 (1:0) | |
| 15:00 | FK Varnsdorf | - | FC Sellier & Bellot Vlašim | 0:1 (0:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sigma Olomouc | 10 | 7 | 3 | 0 | 16:5 | 11 | 24 |
| 2 |
|
Baník Ostrava | 10 | 6 | 4 | 0 | 20:8 | 12 | 22 |
| 3 |
|
Dynamo České Budějovice | 10 | 6 | 2 | 2 | 14:8 | 6 | 20 |
| 4 |
|
SFC Opava | 10 | 5 | 3 | 2 | 20:14 | 6 | 18 |
| 5 |
|
FK Baník Sokolov | 10 | 4 | 4 | 2 | 14:14 | 0 | 16 |
| 6 |
|
1. SC Znojmo | 10 | 4 | 3 | 3 | 19:15 | 4 | 15 |
| 7 |
|
Viktoria Žižkov | 10 | 3 | 5 | 2 | 17:15 | 2 | 14 |
| 8 |
|
FC Sellier & Bellot Vlašim | 10 | 4 | 2 | 4 | 16:14 | 2 | 14 |
| 9 |
|
FK Ústí nad Labem | 10 | 4 | 2 | 4 | 13:13 | 0 | 14 |
| 10 |
|
MFK Frýdek-Místek, a.s. | 10 | 3 | 3 | 4 | 17:18 | -1 | 12 |
| 11 |
|
FK Varnsdorf | 10 | 3 | 3 | 4 | 11:12 | -1 | 12 |
| 12 |
|
FK MAS Táborsko | 10 | 2 | 5 | 3 | 11:13 | -2 | 11 |
| 13 |
|
FK Fotbal Třinec | 10 | 2 | 3 | 5 | 16:21 | -5 | 9 |
| 14 |
|
FK Pardubice | 10 | 1 | 4 | 5 | 6:16 | -10 | 7 |
| 15 |
|
MFK Vítkovice | 10 | 2 | 0 | 8 | 11:21 | -10 | 6 |
| 16 |
|
1. SK Prostějov | 10 | 0 | 2 | 8 | 5:19 | -14 | 2 |
| Promotion | |
| Relegation |
