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| 1st(1st leg) | Viktoria Plzeň | - | Sigma Olomouc | 4:2 (2:0) | ||
| 2nd (2nd leg) | Sigma Olomouc | - | Viktoria Plzeň | 1:1 (1:0) | ||
| 1st(1st leg): 02/03/2016 17:00 | 2nd (2nd leg): 16/03/2016 16:00 | Winner: Viktoria Plzeň | ||||
| 1st(1st leg) | FK Dukla Praha | - | FK Jablonec | 0:0 (0:0) | ||
| 2nd (2nd leg) | FK Jablonec | - | FK Dukla Praha | 2:1 (0:1) | ||
| 1st(1st leg): 03/03/2016 15:00 | 2nd (2nd leg): 15/03/2016 16:30 | Winner: FK Jablonec | ||||
| 1st(1st leg) | Slovan Liberec | - | AC Sparta Praha | 2:1 (1:1) | ||
| 2nd (2nd leg) | AC Sparta Praha | - | Slovan Liberec | 2:0 (1:0) | ||
| 1st(1st leg): 03/03/2016 17:00 | 2nd (2nd leg): 30/03/2016 17:00 | Winner: AC Sparta Praha | ||||
| 1st(1st leg) | FC Hradec Králové | - | FK Mladá Boleslav | 1:4 | ||
| 2nd (2nd leg) | FK Mladá Boleslav | - | FC Hradec Králové | 1:1 (0:0) | ||
| 1st(1st leg): 16/03/2016 16:00 | 2nd (2nd leg): 30/03/2016 14:00 | Winner: FK Mladá Boleslav | ||||
