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| 26/03/2017 | 11:15 | SFC Opava | - | Zbrojovka Brno | 2:1 (0:1) | |
| 04/04/2017 | 19:15 | Bohemians Praha 1905 | - | FK Mladá Boleslav | 3:4 (0:0, 1:1, 1:1) pso | |
| 05/04/2017 | 19:15 | Slovan Liberec | - | FC Fastav Zlín | 2:4 (1:0, 2:2) aet | |
| 12/04/2017 | 17:45 | Slavia Praha | - | MFK Karviná | 5:2 (2:1) |
