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| 1st(1st leg) | Guadix Club de Fútbol | - | Granada CF | 0:0 (0:0) | ||
| 2nd (2nd leg) | Granada CF | - | Guadix Club de Fútbol | 5:4 (0:0, 0:0, 0:0) pso | ||
| 1st(1st leg): 10/01/2001 19:30 | 2nd (2nd leg): 17/01/2001 19:30 | Winner: Granada CF | ||||
| 1st(1st leg) | Racing Santander | - | Athletic Bilbao | 2:0 (2:0) | ||
| 2nd (2nd leg) | Athletic Bilbao | - | Racing Santander | 0:0 (0:0) | ||
| 1st(1st leg): 10/01/2001 19:30 | 2nd (2nd leg): 17/01/2001 19:15 | Winner: Racing Santander | ||||
| 1st(1st leg) | CD Leganés | - | RC Celta | 1:2 (1:1) | ||
| 2nd (2nd leg) | RC Celta | - | CD Leganés | 0:1 (0:0) | ||
| 1st(1st leg): 10/01/2001 20:00 | 2nd (2nd leg): 17/01/2001 20:15 | Winner: RC Celta | ||||
| 1st(1st leg) | CF Extremadura | - | Espanyol Barcelona | 1:1 (0:0) | ||
| 2nd (2nd leg) | Espanyol Barcelona | - | CF Extremadura | 1:0 (0:0) | ||
| 1st(1st leg): 10/01/2001 20:00 | 2nd (2nd leg): 17/01/2001 20:00 | Winner: Espanyol Barcelona | ||||
| 1st(1st leg) | Real Murcia | - | Real Zaragoza | 2:3 (1:0) | ||
| 2nd (2nd leg) | Real Zaragoza | - | Real Murcia | 4:1 (2:0) | ||
| 1st(1st leg): 10/01/2001 20:00 | 2nd (2nd leg): 17/01/2001 19:30 | Winner: Real Zaragoza | ||||
| 1st(1st leg) | Gimnástica Torrelavega | - | FC Barcelona | 0:1 (0:0) | ||
| 2nd (2nd leg) | FC Barcelona | - | Gimnástica Torrelavega | 0:0 (0:0) | ||
| 1st(1st leg): 10/01/2001 20:15 | 2nd (2nd leg): 16/01/2001 20:30 | Winner: FC Barcelona | ||||
| 1st(1st leg) | CD Tenerife | - | RCD Mallorca | 0:2 (0:2) | ||
| 2nd (2nd leg) | RCD Mallorca | - | CD Tenerife | 2:0 (1:0) | ||
| 1st(1st leg): 10/01/2001 21:00 | 2nd (2nd leg): 17/01/2001 20:00 | Winner: RCD Mallorca | ||||
| 1st(1st leg) | Atlético Madrid | - | Rayo Vallecano | 2:2 (2:0) | ||
| 2nd (2nd leg) | Rayo Vallecano | - | Atlético Madrid | 0:2 (0:1) | ||
| 1st(1st leg): 11/01/2001 20:30 | 2nd (2nd leg): 17/01/2001 20:00 | Winner: Atlético Madrid | ||||
