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| 25/08/2012 | 17:00 | FC Brussels | - | RFC Liège | 2:0 (0:0) | |
| 18:00 | KV Oostende | - | UR La Louvière Centre | 2:1 (0:1, 1:1) aet | ||
| 18:30 | KSV Oudenaarde | - | R. White Star Woluwe FC | 1:2 (0:1) | ||
| 19:00 | RU Wallonne Ciney | - | Union La Calamine | 2:0 | ||
| 19:00 | KVC Westerlo | - | KSK Heist | 2:0 | ||
| 19:00 | Royal Mouscron-Péruwelz | - | Spouwen-Mopertingen | 3:0 | ||
| 19:00 | Sint-Truidense VV | - | CS Visé | 4:2 (1:0, 1:1, 1:1) pso | ||
| 19:00 | Hoogstraten VV | - | RFC Tournai | 3:1 | ||
| 19:00 | SK Deinze | - | Olsa Brakel | 2:0 | ||
| 26/08/2012 | 15:00 | KVW Zaventem | - | SV Voorde | 6:1 | |
| 15:00 | RBD Borinage | - | Royal Antwerp FC | 1:0 (0:0, 0:0) aet | ||
| 15:00 | KSC Grimbergen | - | KFC Dessel Sport | 1:2 | ||
| 15:00 | Bocholter VV | - | Excelsior Virton | 2:1 | ||
| 15:00 | Entente Bertrigeoise | - | FC Pepingen | 2:1 | ||
| 15:00 | Union Saint-Gilloise | - | AFC Tubize | 3:1 (1:1) | ||
| 15:00 | KVV Coxyde | - | KAS Eupen | 2:1 |
