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| 1st(1st leg) | Atlético Madrid | - | FC Porto | 2:2 (2:1) | ||
| 2nd (2nd leg) | FC Porto | - | Atlético Madrid | 0:0 (0:0) | ||
| 1st(1st leg): 24/02/2009 19:45 | 2nd (2nd leg): 11/03/2009 19:45 | Winner: FC Porto | ||||
| 1st(1st leg) | Olympique Lyon | - | FC Barcelona | 1:1 (1:0) | ||
| 2nd (2nd leg) | FC Barcelona | - | Olympique Lyon | 5:2 (4:1) | ||
| 1st(1st leg): 24/02/2009 19:45 | 2nd (2nd leg): 11/03/2009 19:45 | Winner: FC Barcelona | ||||
| 1st(1st leg) | Arsenal FC | - | AS Roma | 1:0 (1:0) | ||
| 2nd (2nd leg) | AS Roma | - | Arsenal FC | 6:7 (1:0, 1:0, 1:0) pso | ||
| 1st(1st leg): 24/02/2009 19:45 | 2nd (2nd leg): 11/03/2009 19:45 | Winner: Arsenal FC | ||||
| 1st(1st leg) | Inter | - | Manchester United | 0:0 (0:0) | ||
| 2nd (2nd leg) | Manchester United | - | Inter | 2:0 (1:0) | ||
| 1st(1st leg): 24/02/2009 19:45 | 2nd (2nd leg): 11/03/2009 19:45 | Winner: Manchester United | ||||
| 1st(1st leg) | Chelsea FC | - | Juventus | 1:0 (1:0) | ||
| 2nd (2nd leg) | Juventus | - | Chelsea FC | 2:2 (1:1) | ||
| 1st(1st leg): 25/02/2009 19:45 | 2nd (2nd leg): 10/03/2009 19:45 | Winner: Chelsea FC | ||||
| 1st(1st leg) | Villarreal CF | - | Panathinaikos | 1:1 (0:0) | ||
| 2nd (2nd leg) | Panathinaikos | - | Villarreal CF | 1:2 (0:0) | ||
| 1st(1st leg): 25/02/2009 19:45 | 2nd (2nd leg): 10/03/2009 19:45 | Winner: Villarreal CF | ||||
| 1st(1st leg) | Sporting CP | - | Bayern München | 0:5 (0:1) | ||
| 2nd (2nd leg) | Bayern München | - | Sporting CP | 7:1 (4:1) | ||
| 1st(1st leg): 25/02/2009 19:45 | 2nd (2nd leg): 10/03/2009 19:45 | Winner: Bayern München | ||||
| 1st(1st leg) | Real Madrid | - | Liverpool FC | 0:1 (0:0) | ||
| 2nd (2nd leg) | Liverpool FC | - | Real Madrid | 4:0 (2:0) | ||
| 1st(1st leg): 25/02/2009 19:45 | 2nd (2nd leg): 10/03/2009 19:45 | Winner: Liverpool FC | ||||
