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| 1st(1st leg) | Houston Dynamo | - | Atlante | 1:1 (1:0) | ||
| 2nd (2nd leg) | Atlante | - | Houston Dynamo | 3:0 (2:0) | ||
| 1st(1st leg): 25/02/2009 03:00 | 2nd (2nd leg): 04/03/2009 03:00 | Winner: Atlante | ||||
| 1st(1st leg) | CF Montréal | - | Santos Laguna | 2:0 (1:0) | ||
| 2nd (2nd leg) | Santos Laguna | - | CF Montréal | 5:2 (1:2) | ||
| 1st(1st leg): 26/02/2009 01:00 | 2nd (2nd leg): 06/03/2009 03:00 | Winner: Santos Laguna | ||||
| 1st(1st leg) | Cruz Azul | - | Pumas UNAM | 1:0 (1:0) | ||
| 2nd (2nd leg) | Pumas UNAM | - | Cruz Azul | 0:1 (0:0) | ||
| 1st(1st leg): 26/02/2009 03:00 | 2nd (2nd leg): 05/03/2009 03:00 | Winner: Cruz Azul | ||||
| 1st(1st leg) | Puerto Rico Islanders | - | Marathón | 2:1 (0:1) | ||
| 2nd (2nd leg) | Marathón | - | Puerto Rico Islanders | 0:1 (0:0) | ||
| 1st(1st leg): 27/02/2009 01:00 | 2nd (2nd leg): 05/03/2009 01:00 | Winner: Puerto Rico Islanders | ||||
