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| 07/11/2020 | 13:00 | FK Ústí nad Labem | - | Viktoria Žižkov | 2:1 (0:1) | |
| 13:00 | FK Fotbal Třinec | - | FC Hradec Králové | 1:2 (0:2) | ||
| 13:00 | SK Líšeň | - | MFK Chrudim | 1:0 (1:0) | ||
| 13:00 | 1. SK Prostějov | - | Slavoj Vyšehrad | 3:0 (2:0) | ||
| 08/11/2020 | 09:15 | FK Dukla Praha | - | Vysočina Jihlava | 3:3 (2:0) | |
| 10:00 | FK Blansko | - | FK MAS Táborsko | 0:2 (0:1) | ||
| 13:00 | FK Varnsdorf | - | FC Sellier & Bellot Vlašim | 0:2 (0:1) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 7 | 5 | 2 | 0 | 11:5 | 6 | 17 |
| 2 |
|
SK Líšeň | 7 | 4 | 3 | 0 | 13:7 | 6 | 15 |
| 3 |
|
FK Ústí nad Labem | 7 | 4 | 2 | 1 | 12:8 | 4 | 14 |
| 4 |
|
1. SK Prostějov | 7 | 3 | 3 | 1 | 10:6 | 4 | 12 |
| 5 |
|
Viktoria Žižkov | 7 | 4 | 0 | 3 | 11:8 | 3 | 12 |
| 6 |
|
FK Dukla Praha | 7 | 2 | 4 | 1 | 13:9 | 4 | 10 |
| 7 |
|
MFK Chrudim | 7 | 3 | 1 | 3 | 8:5 | 3 | 10 |
| 8 |
|
Vysočina Jihlava | 7 | 2 | 2 | 3 | 15:13 | 2 | 8 |
| 9 |
|
FC Sellier & Bellot Vlašim | 7 | 2 | 2 | 3 | 13:12 | 1 | 8 |
| 10 |
|
FK MAS Táborsko | 7 | 2 | 2 | 3 | 7:8 | -1 | 8 |
| 11 |
|
FK Varnsdorf | 7 | 1 | 4 | 2 | 5:8 | -3 | 7 |
| 12 |
|
FK Blansko | 7 | 1 | 3 | 3 | 7:12 | -5 | 6 |
| 13 |
|
FK Fotbal Třinec | 7 | 1 | 1 | 5 | 9:17 | -8 | 4 |
| 14 |
|
Slavoj Vyšehrad | 7 | 0 | 1 | 6 | 1:17 | -16 | 1 |
| Promotion | |
| Relegation |
