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| 05/05/2010 | 16:30 | 1. FK Příbram | - | Bohemians 1905 | 2:0 (2:0) | |
| 16:30 | SK Kladno | - | Baník Ostrava | 0:3 (0:1) | ||
| 16:30 | AC Sparta Praha | - | 1. FC Slovácko | 4:0 (2:0) | ||
| 16:30 | FK Mladá Boleslav | - | Dynamo České Budějovice | 0:1 (0:1) | ||
| 16:30 | Sigma Olomouc | - | Slovan Liberec | 2:0 (2:0) | ||
| 16:30 | Viktoria Plzeň | - | Slavia Praha | 4:2 (1:2) | ||
| 16:30 | 1. FC Brno | - | Bohemians Praha | 0:0 (0:0) | ||
| 19:00 | FK Baumit Jablonec | - | FK Teplice | 0:0 (0:0) |
| » Tables | Home | Away | First round | Second round | First half | Second half |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
AC Sparta Praha | 28 | 15 | 13 | 0 | 39:12 | 27 | 58 |
| 2 |
|
Baník Ostrava | 28 | 17 | 7 | 4 | 44:22 | 22 | 58 |
| 3 |
|
FK Baumit Jablonec | 28 | 16 | 7 | 5 | 37:24 | 13 | 55 |
| 4 |
|
FK Teplice | 28 | 15 | 9 | 4 | 43:23 | 20 | 54 |
| 5 |
|
Viktoria Plzeň | 28 | 12 | 10 | 6 | 38:29 | 9 | 46 |
| 6 |
|
Sigma Olomouc | 28 | 13 | 4 | 11 | 45:35 | 10 | 43 |
| 7 |
|
FK Mladá Boleslav | 28 | 11 | 5 | 12 | 45:38 | 7 | 38 |
| 8 |
|
Slavia Praha | 28 | 10 | 8 | 10 | 36:32 | 4 | 38 |
| 9 |
|
1. FK Příbram | 28 | 10 | 5 | 13 | 33:38 | -5 | 35 |
| 10 |
|
Slovan Liberec | 28 | 9 | 6 | 13 | 30:36 | -6 | 33 |
| 11 |
|
1. FC Brno | 28 | 8 | 8 | 12 | 27:37 | -10 | 32 |
| 12 |
|
Dynamo České Budějovice | 28 | 7 | 10 | 11 | 23:30 | -7 | 31 |
| 13 |
|
Bohemians 1905 | 28 | 7 | 9 | 12 | 19:28 | -9 | 30 |
| 14 |
|
1. FC Slovácko | 28 | 7 | 5 | 16 | 24:39 | -15 | 26 |
| 15 |
|
SK Kladno | 28 | 7 | 3 | 18 | 23:48 | -25 | 24 |
| 16 |
|
Bohemians Praha | 28 | 4 | 3 | 21 | 25:60 | -35 | -5 |
| Champions League Quali. | |
| Europa League | |
| Relegation |
