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| 1st(1st leg) | Slovan Liberec | - | Sigma Olomouc | 0:2 | ||
| 2nd (2nd leg) | Sigma Olomouc | - | Slovan Liberec | 1:0 | ||
| 1st(1st leg): 31/03/2010 17:00 | 2nd (2nd leg): 07/04/2010 17:00 | Winner: Sigma Olomouc | ||||
| 1st(1st leg) | FK Teplice | - | FK Baumit Jablonec | 1:3 | ||
| 2nd (2nd leg) | FK Baumit Jablonec | - | FK Teplice | 0:0 | ||
| 1st(1st leg): 31/03/2010 17:00 | 2nd (2nd leg): 08/04/2010 16:30 | Winner: FK Baumit Jablonec | ||||
| 1st(1st leg) | Viktoria Plzeň | - | 1. FK Příbram | 1:0 | ||
| 2nd (2nd leg) | 1. FK Příbram | - | Viktoria Plzeň | 1:1 | ||
| 1st(1st leg): 31/03/2010 17:00 | 2nd (2nd leg): 08/04/2010 16:00 | Winner: Viktoria Plzeň | ||||
| 1st(1st leg) | Slavia Praha | - | AC Sparta Praha | 1:0 | ||
| 2nd (2nd leg) | AC Sparta Praha | - | Slavia Praha | 0:1 | ||
| 1st(1st leg): 01/04/2010 18:00 | 2nd (2nd leg): 15/04/2010 19:00 | Winner: Slavia Praha | ||||
