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| 16/09/2009 | 16:00 | Brønshøj BK | - | FC Midtjylland | 1:3 | |
| 23/09/2009 | 15:00 | Blokhus FC | - | Brøndby IF | 0:2 | |
| 15:30 | Tved BK | - | HB Køge | 0:2 | ||
| 15:30 | Aarhus Fremad | - | Brabrand IF | 3:4 pso | ||
| 15:30 | Rishøj BK | - | Viborg FF | 0:4 | ||
| 15:30 | Frederiksberg BK | - | Vejle BK | 0:4 | ||
| 15:30 | Allerød FK | - | Hobro IK | 0:2 | ||
| 15:30 | AIK 65 Strøby | - | Tårnby FF | 5:4 | ||
| 15:30 | Marstal/Rise | - | Silkeborg IF | 1:3 | ||
| 15:30 | Vejen SF | - | Randers FC | 0:9 | ||
| 17:00 | Elite 3000 | - | FC København | 1:2 | ||
| 18:00 | Akademisk BK | - | FC Nordsjælland | 1:4 | ||
| 18:00 | Hellerup IK | - | SønderjyskE | 2:4 | ||
| 18:00 | Thisted FC | - | Aalborg BK | 0:3 | ||
| 18:00 | FC Fredericia | - | Odense BK | 3:5 | ||
| 01/10/2009 | 18:00 | Lyngby BK | - | Esbjerg fB | 1:2 |
