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| 1st(1st leg) | FC Barcelona | - | Sevilla FC | 1:2 (0:0) | ||
| 2nd (2nd leg) | Sevilla FC | - | FC Barcelona | 0:1 (0:0) | ||
| 1st(1st leg): 05/01/2010 21:00 | 2nd (2nd leg): 13/01/2010 21:00 | Winner: Sevilla FC | ||||
| 1st(1st leg) | RC Celta | - | Villarreal CF | 1:1 (1:1) | ||
| 2nd (2nd leg) | Villarreal CF | - | RC Celta | 0:1 (0:0) | ||
| 1st(1st leg): 06/01/2010 17:00 | 2nd (2nd leg): 12/01/2010 20:00 | Winner: RC Celta | ||||
| 1st(1st leg) | AD Alcorcón | - | Racing Santander | 2:3 (0:1) | ||
| 2nd (2nd leg) | Racing Santander | - | AD Alcorcón | 0:0 (0:0) | ||
| 1st(1st leg): 06/01/2010 19:00 | 2nd (2nd leg): 13/01/2010 19:00 | Winner: Racing Santander | ||||
| 1st(1st leg) | Valencia CF | - | Deportivo La Coruña | 1:2 (0:0) | ||
| 2nd (2nd leg) | Deportivo La Coruña | - | Valencia CF | 2:2 (0:2) | ||
| 1st(1st leg): 06/01/2010 19:00 | 2nd (2nd leg): 13/01/2010 19:00 | Winner: Deportivo La Coruña | ||||
| 1st(1st leg) | Recreativo Huelva | - | Atlético Madrid | 3:0 (2:0) | ||
| 2nd (2nd leg) | Atlético Madrid | - | Recreativo Huelva | 5:1 (3:0) | ||
| 1st(1st leg): 06/01/2010 21:00 | 2nd (2nd leg): 14/01/2010 21:00 | Winner: Atlético Madrid | ||||
| 1st(1st leg) | Hércules CF | - | CA Osasuna | 2:1 (1:0) | ||
| 2nd (2nd leg) | CA Osasuna | - | Hércules CF | 1:0 (0:0) | ||
| 1st(1st leg): 07/01/2010 19:00 | 2nd (2nd leg): 13/01/2010 19:00 | Winner: CA Osasuna | ||||
| 1st(1st leg) | Rayo Vallecano | - | RCD Mallorca | 2:1 (2:1) | ||
| 2nd (2nd leg) | RCD Mallorca | - | Rayo Vallecano | 3:1 (0:1) | ||
| 1st(1st leg): 07/01/2010 19:00 | 2nd (2nd leg): 14/01/2010 19:00 | Winner: RCD Mallorca | ||||
| 1st(1st leg) | Málaga CF | - | Getafe CF | 2:1 (2:0) | ||
| 2nd (2nd leg) | Getafe CF | - | Málaga CF | 5:1 (2:0) | ||
| 1st(1st leg): 07/01/2010 21:00 | 2nd (2nd leg): 13/01/2010 21:00 | Winner: Getafe CF | ||||
