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| 1st(1st leg) | Lleida Esportiu | - | Barakaldo CF | 1:0 (1:0) | ||
| 2nd (2nd leg) | Barakaldo CF | - | Lleida Esportiu | 1:1 (0:0) | ||
| 1st(1st leg): 21/05/2016 17:30 | 2nd (2nd leg): 29/05/2016 19:00 | Winner: Lleida Esportiu | ||||
| 1st(1st leg) | Sevilla Atlético | - | UD Socuéllamos | 1:1 (1:1) | ||
| 2nd (2nd leg) | UD Socuéllamos | - | Sevilla Atlético | 1:2 (0:0) | ||
| 1st(1st leg): 21/05/2016 18:00 | 2nd (2nd leg): 29/05/2016 16:15 | Winner: Sevilla Atlético | ||||
| 1st(1st leg) | Cádiz CF | - | Racing Ferrol | 0:0 (0:0) | ||
| 2nd (2nd leg) | Racing Ferrol | - | Cádiz CF | 1:2 (0:2) | ||
| 1st(1st leg): 21/05/2016 20:00 | 2nd (2nd leg): 29/05/2016 16:00 | Winner: Cádiz CF | ||||
| 1st(1st leg) | CD Toledo | - | Real Murcia | 0:0 (0:0) | ||
| 2nd (2nd leg) | Real Murcia | - | CD Toledo | 1:2 (0:1) | ||
| 1st(1st leg): 22/05/2016 16:30 | 2nd (2nd leg): 29/05/2016 17:00 | Winner: CD Toledo | ||||
| 1st(1st leg) | CD Tudelano | - | Hércules CF | 0:1 (0:0) | ||
| 2nd (2nd leg) | Hércules CF | - | CD Tudelano | 1:0 (1:0) | ||
| 1st(1st leg): 22/05/2016 17:00 | 2nd (2nd leg): 29/05/2016 18:30 | Winner: Hércules CF | ||||
| 1st(1st leg) | UD Logroñés | - | Villarreal CF B | 0:0 (0:0) | ||
| 2nd (2nd leg) | Villarreal CF B | - | UD Logroñés | 1:1 (1:1) | ||
| 1st(1st leg): 22/05/2016 17:30 | 2nd (2nd leg): 28/05/2016 17:00 | Winner: UD Logroñés | ||||
