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| 1st(1st leg) | Shakhtar Donetsk | - | RSC Anderlecht | 3:1 (2:0) | ||
| 2nd (2nd leg) | RSC Anderlecht | - | Shakhtar Donetsk | 0:1 (0:0) | ||
| 1st(1st leg): 10/03/2016 18:00 | 2nd (2nd leg): 17/03/2016 20:05 | Winner: Shakhtar Donetsk | ||||
| 1st(1st leg) | FC Basel | - | Sevilla FC | 0:0 (0:0) | ||
| 2nd (2nd leg) | Sevilla FC | - | FC Basel | 3:0 (3:0) | ||
| 1st(1st leg): 10/03/2016 18:00 | 2nd (2nd leg): 17/03/2016 20:05 | Winner: Sevilla FC | ||||
| 1st(1st leg) | Borussia Dortmund | - | Tottenham Hotspur | 3:0 (1:0) | ||
| 2nd (2nd leg) | Tottenham Hotspur | - | Borussia Dortmund | 1:2 (0:1) | ||
| 1st(1st leg): 10/03/2016 18:00 | 2nd (2nd leg): 17/03/2016 20:05 | Winner: Borussia Dortmund | ||||
| 1st(1st leg) | Fenerbahçe | - | Sporting Braga | 1:0 (0:0) | ||
| 2nd (2nd leg) | Sporting Braga | - | Fenerbahçe | 4:1 (1:1) | ||
| 1st(1st leg): 10/03/2016 18:00 | 2nd (2nd leg): 17/03/2016 20:05 | Winner: Sporting Braga | ||||
| 1st(1st leg) | Villarreal CF | - | Bayer Leverkusen | 2:0 (1:0) | ||
| 2nd (2nd leg) | Bayer Leverkusen | - | Villarreal CF | 0:0 (0:0) | ||
| 1st(1st leg): 10/03/2016 20:05 | 2nd (2nd leg): 17/03/2016 18:00 | Winner: Villarreal CF | ||||
| 1st(1st leg) | Athletic Bilbao | - | Valencia CF | 1:0 (1:0) | ||
| 2nd (2nd leg) | Valencia CF | - | Athletic Bilbao | 2:1 (2:0) | ||
| 1st(1st leg): 10/03/2016 20:05 | 2nd (2nd leg): 17/03/2016 18:00 | Winner: Athletic Bilbao | ||||
| 1st(1st leg) | Liverpool FC | - | Manchester United | 2:0 (1:0) | ||
| 2nd (2nd leg) | Manchester United | - | Liverpool FC | 1:1 (1:1) | ||
| 1st(1st leg): 10/03/2016 20:05 | 2nd (2nd leg): 17/03/2016 20:05 | Winner: Liverpool FC | ||||
| 1st(1st leg) | AC Sparta Praha | - | Lazio Roma | 1:1 (1:1) | ||
| 2nd (2nd leg) | Lazio Roma | - | AC Sparta Praha | 0:3 (0:3) | ||
| 1st(1st leg): 10/03/2016 20:05 | 2nd (2nd leg): 17/03/2016 18:00 | Winner: AC Sparta Praha | ||||
