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| 24/09/2013 | 15:00 | ŠKF Sereď | - | MFK Košice | 2:3 (0:0, 0:0) pso | |
| 15:00 | MŠK Rimavská Sobota | - | FK Senica | 5:6 (0:1, 1:1) pso | ||
| 15:00 | 1. FC Tatran Prešov | - | FC DAC 1904 | 1:0 (0:0) | ||
| 15:00 | Zlaté Moravce | - | Slovan Duslo Šaľa | 3:2 (2:0) | ||
| 15:00 | Spartak Trnava | - | Tatran Liptovský Mikuláš | 3:0 (2:0) | ||
| 15:30 | MFK Ružomberok | - | MFK Zemplín Michalovce | 5:4 (0:0, 0:0) pso | ||
| 25/09/2013 | 15:00 | OFK Dunajská Lužná | - | Slovan Bratislava | 0:3 (0:1) | |
| 15:00 | FK Slovan Nemšová | - | MŠK Žilina | 0:1 (0:1) |
