1st(1st leg) Slavia Praha - 1. FC Kaiserslautern 0:0 (0:0)
2nd (2nd leg) 1. FC Kaiserslautern - Slavia Praha 1:0 (0:0)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: 1. FC Kaiserslautern
1st(1st leg) CD Alavés - Inter 3:3 (1:1)
2nd (2nd leg) Inter - CD Alavés 0:2 (0:0)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: CD Alavés
1st(1st leg) PSV Eindhoven - Parma AC 2:1 (1:0)
2nd (2nd leg) Parma AC - PSV Eindhoven 3:2 (0:2)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: PSV Eindhoven
1st(1st leg) AEK Athen - FC Barcelona 0:1 (0:1)
2nd (2nd leg) FC Barcelona - AEK Athen 5:0 (2:0)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: FC Barcelona
1st(1st leg) VfB Stuttgart - RC Celta 0:0 (0:0)
2nd (2nd leg) RC Celta - VfB Stuttgart 2:1 (1:1)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: RC Celta
1st(1st leg) Rayo Vallecano - Girondins Bordeaux 4:1 (1:1)
2nd (2nd leg) Girondins Bordeaux - Rayo Vallecano 1:2 (1:1)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: Rayo Vallecano
1st(1st leg) AS Roma - Liverpool FC 0:2 (0:0)
2nd (2nd leg) Liverpool FC - AS Roma 0:1 (0:0)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: Liverpool FC
1st(1st leg) FC Porto - FC Nantes 3:1 (1:1)
2nd (2nd leg) FC Nantes - FC Porto 2:1 (0:1)
1st(1st leg): 15/02/2001 2nd (2nd leg): 22/02/2001 Winner: FC Porto