| 1. Round | 24/07/2023 | Zbrojovka Brno | - | FC Sellier & Bellot Vlašim | 1:1 |  |
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| 2. Round | 29/07/2023 | FC Sellier & Bellot Vlašim | - | MFK Chrudim | 1:1 |  |
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| 3. Round | 02/08/2023 | FK Varnsdorf | - | FC Sellier & Bellot Vlašim | 3:3 | |
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| 4. Round | 05/08/2023 | FC Sellier & Bellot Vlašim | - | MFK Vyškov | 1:1 | |
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| 5. Round | 12/08/2023 | 1. SK Prostějov | - | FC Sellier & Bellot Vlašim | 0:1 | |
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| 6. Round | 19/08/2023 | FC Sellier & Bellot Vlašim | - | FK Příbram | 3:1 | |
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| 7. Round | 27/08/2023 | Viktoria Žižkov | - | FC Sellier & Bellot Vlašim | 0:1 | |
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| 9. Round | 16/09/2023 | SK Líšeň | - | FC Sellier & Bellot Vlašim | 2:2 | |
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| 10. Round | 23/09/2023 | FC Sellier & Bellot Vlašim | - | SFC Opava | 1:1 | ||||
| 11. Round | 30/09/2023 | Hanácká Slávia Kroměříž | - | FC Sellier & Bellot Vlašim | 2:1 | |
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| 12. Round | 07/10/2023 | FC Sellier & Bellot Vlašim | - | FK Dukla Praha | 3:2 | |
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| 13. Round | 22/10/2023 | Sigma Olomouc B | - | FC Sellier & Bellot Vlašim | 1:0 | ||||
| 14. Round | 28/10/2023 | FK MAS Táborsko | - | FC Sellier & Bellot Vlašim | 1:1 | |
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| 15. Round | 04/11/2023 | FC Sellier & Bellot Vlašim | - | AC Sparta Praha B | 0:1 | |
Gambia