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| 30/05/2021 | 14:00 | FK Blansko | - | 1. SK Prostějov | 2:1 (0:0) | |
| 14:00 | FK Dukla Praha | - | Slavoj Vyšehrad | 3:0 (2:0) | ||
| 14:00 | Vysočina Jihlava | - | MFK Chrudim | 3:1 (2:1) | ||
| 14:00 | FK Varnsdorf | - | FK Ústí nad Labem | 0:1 (0:0) | ||
| 14:00 | SK Líšeň | - | FK Fotbal Třinec | 1:1 (1:1) | ||
| 14:00 | Viktoria Žižkov | - | FK MAS Táborsko | 2:0 (0:0) | ||
| 14:00 | FC Sellier & Bellot Vlašim | - | FC Hradec Králové | 2:2 (1:1) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 26 | 17 | 7 | 2 | 51:22 | 29 | 58 |
| 2 |
|
SK Líšeň | 26 | 13 | 11 | 2 | 43:24 | 19 | 50 |
| 3 |
|
1. SK Prostějov | 26 | 12 | 7 | 7 | 40:35 | 5 | 43 |
| 4 |
|
Viktoria Žižkov | 26 | 13 | 3 | 10 | 42:38 | 4 | 42 |
| 5 |
|
FK Ústí nad Labem | 26 | 12 | 5 | 9 | 24:29 | -5 | 41 |
| 6 |
|
FC Sellier & Bellot Vlašim | 26 | 10 | 7 | 9 | 38:33 | 5 | 37 |
| 7 |
|
Vysočina Jihlava | 26 | 9 | 8 | 9 | 44:44 | 0 | 35 |
| 8 |
|
FK Dukla Praha | 26 | 9 | 7 | 10 | 36:30 | 6 | 34 |
| 9 |
|
FK Fotbal Třinec | 26 | 9 | 6 | 11 | 32:33 | -1 | 33 |
| 10 |
|
MFK Chrudim | 26 | 9 | 4 | 13 | 33:36 | -3 | 31 |
| 11 |
|
FK MAS Táborsko | 26 | 8 | 7 | 11 | 25:28 | -3 | 31 |
| 12 |
|
FK Varnsdorf | 26 | 6 | 12 | 8 | 20:27 | -7 | 30 |
| 13 |
|
FK Blansko | 26 | 7 | 6 | 13 | 30:34 | -4 | 27 |
| 14 |
|
Slavoj Vyšehrad | 26 | 1 | 4 | 21 | 15:60 | -45 | 7 |
| Promotion | |
| Relegation |
