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| 10/08/2010 | 19:30 | Stenhousemuir FC | - | Dundee FC | 4:1 (2:0) | |
| 19:30 | Queen's Park FC | - | Forfar Athletic | 2:3 (1:0, 2:2) aet | ||
| 19:45 | Dunfermline Athletic | - | Queen Of The South | 5:6 (0:0, 1:1, 1:1) pso | ||
| 19:45 | East Fife FC | - | Stirling Albion FC | 3:1 (1:1) | ||
| 19:45 | Partick Thistle | - | Berwick Rangers | 2:1 (1:1) | ||
| 19:45 | Peterhead FC | - | East Stirlingshire FC | 6:1 (1:1) | ||
| 19:45 | Ross County FC | - | Greenock Morton FC | 3:1 (1:1) | ||
| 19:45 | Ayr United | - | Cowdenbeath FC | 2:0 (0:0) |
