1st(1st leg) Alma KTZH - AC Sparta Praha 1:0 (0:0)
2nd (2nd leg) AC Sparta Praha - Alma KTZH 2:0 (2:0)
1st(1st leg): 30/09/2009 10:00 2nd (2nd leg): 07/10/2009 14:00 Winner: AC Sparta Praha
1st(1st leg) WFC Zhytlobud-1 Kharkiv - Umeå IK FF 0:5 (0:3)
2nd (2nd leg) Umeå IK FF - WFC Zhytlobud-1 Kharkiv 6:0 (3:0)
1st(1st leg): 30/09/2009 13:00 2nd (2nd leg): 07/10/2009 18:00 Winner: Umeå IK FF
1st(1st leg) ASD Torres Calcio - Valur Reykjavik 4:1 (2:0)
2nd (2nd leg) Valur Reykjavik - ASD Torres Calcio 1:2 (0:1)
1st(1st leg): 30/09/2009 14:00 2nd (2nd leg): 07/10/2009 16:30 Winner: ASD Torres Calcio
1st(1st leg) Unia Racibórz - SV Neulengbach 1:3 (0:2)
2nd (2nd leg) SV Neulengbach - Unia Racibórz 0:1 (0:0)
1st(1st leg): 30/09/2009 15:00 2nd (2nd leg): 07/10/2009 15:00 Winner: SV Neulengbach
1st(1st leg) SFK 2000 Sarajevo - Zvezda 2005 Perm 0:3 (0:2)
2nd (2nd leg) Zvezda 2005 Perm - SFK 2000 Sarajevo 5:0 (4:0)
1st(1st leg): 30/09/2009 15:00 2nd (2nd leg): 07/10/2009 14:00 Winner: Zvezda 2005 Perm
1st(1st leg) ŽFK Mašinac Niš - Olympique Lyonnais 0:1 (0:1)
2nd (2nd leg) Olympique Lyonnais - ŽFK Mašinac Niš 5:0 (0:0)
1st(1st leg): 30/09/2009 15:30 2nd (2nd leg): 07/10/2009 18:00 Winner: Olympique Lyonnais
1st(1st leg) Røa IL - Everton LFC 3:0 (2:0)
2nd (2nd leg) Everton LFC - Røa IL 2:0 (1:0)
1st(1st leg): 30/09/2009 15:30 2nd (2nd leg): 07/10/2009 15:30 Winner: Røa IL
1st(1st leg) Universitet Vitebsk - FCR 2001 Duisburg 1:5 (0:4)
2nd (2nd leg) FCR 2001 Duisburg - Universitet Vitebsk 6:3 (5:2)
1st(1st leg): 30/09/2009 16:30 2nd (2nd leg): 07/10/2009 18:30 Winner: FCR 2001 Duisburg
1st(1st leg) Standard Liège - Montpellier HSC 0:0 (0:0)
2nd (2nd leg) Montpellier HSC - Standard Liège 3:1 (0:1)
1st(1st leg): 30/09/2009 17:00 2nd (2nd leg): 07/10/2009 17:00 Winner: Montpellier HSC
1st(1st leg) Viktória Szombathely - Bayern München 0:5 (0:4)
2nd (2nd leg) Bayern München - Viktória Szombathely 4:2 (1:1)
1st(1st leg): 30/09/2009 17:00 2nd (2nd leg): 07/10/2009 17:00 Winner: Bayern München
1st(1st leg) FC Honka - Turbine Potsdam 1:8 (0:4)
2nd (2nd leg) Turbine Potsdam - FC Honka 8:0 (3:0)
1st(1st leg): 30/09/2009 17:00 2nd (2nd leg): 07/10/2009 18:00 Winner: Turbine Potsdam
1st(1st leg) PAOK Saloniki - Arsenal LFC 0:9 (0:5)
2nd (2nd leg) Arsenal LFC - PAOK Saloniki 9:0 (5:0)
1st(1st leg): 30/09/2009 17:00 2nd (2nd leg): 07/10/2009 15:00 Winner: Arsenal LFC
1st(1st leg) FC Zürich Frauen - Linköpings FC 0:2 (0:2)
2nd (2nd leg) Linköpings FC - FC Zürich Frauen 3:0 (2:0)
1st(1st leg): 30/09/2009 17:00 2nd (2nd leg): 07/10/2009 17:00 Winner: Linköpings FC
1st(1st leg) Fortuna Hjørring - ASD Bardolino 4:0 (1:0)
2nd (2nd leg) ASD Bardolino - Fortuna Hjørring 2:1 (1:1)
1st(1st leg): 30/09/2009 18:00 2nd (2nd leg): 07/10/2009 18:30 Winner: Fortuna Hjørring
1st(1st leg) AZ Alkmaar - Brøndby IF 1:2 (1:2)
2nd (2nd leg) Brøndby IF - AZ Alkmaar 1:1 (0:1)
1st(1st leg): 30/09/2009 18:30 2nd (2nd leg): 07/10/2009 18:00 Winner: Brøndby IF
1st(1st leg) Rayo Vallecano - WFC Rossiyanka 1:3 (0:2)
2nd (2nd leg) WFC Rossiyanka - Rayo Vallecano 2:1 (1:0)
1st(1st leg): 30/09/2009 19:30 2nd (2nd leg): 07/10/2009 12:00 Winner: WFC Rossiyanka