| 25. Round | 23/01/2021 | Morecambe FC | - | Colchester United | 3:0 | ||||
| 26. Round | 26/01/2021 | Stevenage FC | - | Colchester United | 0:0 | ||||
| 27. Round | 29/01/2021 | Colchester United | - | Scunthorpe United | 0:1 | ||||
| 29. Round | 14/02/2021 | Colchester United | - | Mansfield Town | 2:2 | ||||
| 30. Round | 20/02/2021 | Crawley Town | - | Colchester United | 1:0 | ||||
| 31. Round | 23/02/2021 | Colchester United | - | Exeter City | 1:2 |  |
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| 32. Round | 27/02/2021 | Forest Green Rovers | - | Colchester United | 3:0 |  |
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| 33. Round | 02/03/2021 | Colchester United | - | Carlisle United | 2:1 | ||||
| 34. Round | 06/03/2021 | Colchester United | - | Newport County | 0:2 | ||||
| 35. Round | 09/03/2021 | Harrogate Town | - | Colchester United | 3:0 | ||||
| 36. Round | 13/03/2021 | Grimsby Town | - | Colchester United | 0:0 | ||||
| 37. Round | 20/03/2021 | Colchester United | - | Port Vale FC | 0:1 | ||||
| 44. Round | 24/04/2021 | Cheltenham Town | - | Colchester United | 1:0 |
England
Nigeria