| 5. Round | 04/09/2010 | Tranmere Rovers | - | Peterborough United | 1:0 |  |
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| 6. Round | 11/09/2010 | Yeovil Town | - | Tranmere Rovers | 3:1 | |
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| 7. Round | 18/09/2010 | Tranmere Rovers | - | Charlton Athletic | 1:1 | ||||
| 8. Round | 25/09/2010 | Colchester United | - | Tranmere Rovers | 3:1 | |
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| 9. Round | 28/09/2010 | Bristol Rovers | - | Tranmere Rovers | 0:1 | ||||
| 10. Round | 02/10/2010 | Tranmere Rovers | - | Brighton & Hove Albion | 1:1 | ||||
| 11. Round | 09/10/2010 | Southampton FC | - | Tranmere Rovers | 2:0 | ||||
| 12. Round | 16/10/2010 | Tranmere Rovers | - | Brentford FC | 0:3 | ||||
| 13. Round | 23/10/2010 | Walsall FC | - | Tranmere Rovers | 1:4 | ||||
| 16. Round | 13/11/2010 | Tranmere Rovers | - | Plymouth Argyle | 1:0 | |
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| 17. Round | 20/11/2010 | Notts County | - | Tranmere Rovers | 0:1 | ||||
| 18. Round | 23/11/2010 | Tranmere Rovers | - | Hartlepool United | 0:1 | |
France