| 1. Round | 31/01/2010 | Club Rubio Ñu | - | Club Olimpia | 1:0 | ||||
| 2. Round | 06/02/2010 | Libertad | - | Club Rubio Ñu | 2:1 | ||||
| 4. Round | 17/02/2010 | Club Rubio Ñu | - | Sol de América | 5:0 |  |
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| 5. Round | 21/02/2010 | Tacuary | - | Club Rubio Ñu | 0:1 | ||||
| 6. Round | 01/03/2010 | Club Rubio Ñu | - | Club Nacional | 4:1 | ||||
| 7. Round | 07/03/2010 | Sportivo Trinidense | - | Club Rubio Ñu | 1:1 | ||||
| 8. Round | 13/03/2010 | Club Rubio Ñu | - | Sportivo Luqueño | 0:0 |  |
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| 10. Round | 21/03/2010 | Club Rubio Ñu | - | Sport Colombia | 2:1 | ||||
| 12. Round | 04/04/2010 | Club Olimpia | - | Club Rubio Ñu | 1:1 | ||||
| 13. Round | 10/04/2010 | Club Rubio Ñu | - | Libertad | 2:1 | |
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| 15. Round | 17/04/2010 | Sol de América | - | Club Rubio Ñu | 0:1 | |
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| 16. Round | 25/04/2010 | Club Rubio Ñu | - | Tacuary | 3:1 | |
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| 17. Round | 01/05/2010 | Club Nacional | - | Club Rubio Ñu | 0:0 | ||||
| 18. Round | 08/05/2010 | Club Rubio Ñu | - | Sportivo Trinidense | 1:1 | |
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| 19. Round | 13/05/2010 | Sportivo Luqueño | - | Club Rubio Ñu | 2:2 |
Paraguay