| 1. Round | 08/08/2009 | Rotherham United | - | Accrington Stanley | 1:0 | ||||
| 2. Round | 15/08/2009 | Accrington Stanley | - | Lincoln City | 1:0 | ||||
| 8. Round | 19/09/2009 | Hereford United | - | Accrington Stanley | 2:0 | ||||
| 12. Round | 10/10/2009 | Accrington Stanley | - | Cheltenham Town | 4:0 | ||||
| 15. Round | 30/10/2009 | Grimsby Town | - | Accrington Stanley | 2:2 |  |
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| 16. Round | 14/11/2009 | Accrington Stanley | - | Dagenham & Redbridge | 0:1 | ||||
| 28. Round | 09/02/2010 | Northampton Town | - | Accrington Stanley | 4:0 | ||||
| 33. Round | 23/03/2010 | Accrington Stanley | - | Burton Albion | 0:2 | ||||
| 38. Round | 27/03/2010 | AFC Bournemouth | - | Accrington Stanley | 2:0 |
England