| 1. Round | 01/08/2009 | Willem II | - | Vitesse | 3:1 |  |
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| 3. Round | 15/08/2009 | Sparta Rotterdam | - | Willem II | 2:1 | ||||
| 4. Round | 22/08/2009 | Willem II | - | Heracles Almelo | 0:1 | ||||
| 5. Round | 29/08/2009 | AZ Alkmaar | - | Willem II | 2:1 | ||||
| 6. Round | 13/09/2009 | Willem II | - | Feyenoord | 2:3 | ||||
| 7. Round | 18/09/2009 | Willem II | - | FC Groningen | 2:1 | |
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| 11. Round | 25/10/2009 | Willem II | - | sc Heerenveen | 4:1 | ||||
| 12. Round | 01/11/2009 | RKC Waalwijk | - | Willem II | 0:1 | |
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| 13. Round | 08/11/2009 | NAC Breda | - | Willem II | 4:0 |  |
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| 14. Round | 22/11/2009 | Willem II | - | ADO Den Haag | 1:1 | ||||
| 15. Round | 28/11/2009 | Willem II | - | FC Twente | 1:3 | |
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| 16. Round | 04/12/2009 | VVV-Venlo | - | Willem II | 2:1 | ||||
| 17. Round | 13/12/2009 | Willem II | - | Roda JC Kerkrade | 1:3 | |
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| 18. Round | 19/12/2009 | Feyenoord | - | Willem II | 1:0 | |
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| 19. Round | 23/01/2010 | sc Heerenveen | - | Willem II | 4:2 | ||||
| 20. Round | 29/01/2010 | Willem II | - | RKC Waalwijk | 2:1 | ||||
| 21. Round | 17/02/2010 | NEC Nijmegen | - | Willem II | 2:1 | ||||
| 26. Round | 06/03/2010 | Heracles Almelo | - | Willem II | 3:2 | ||||
| 27. Round | 14/03/2010 | Willem II | - | Sparta Rotterdam | 3:1 | ||||
| 28. Round | 20/03/2010 | Willem II | - | FC Utrecht | 0:3 |
Belgium