| 6. Round | 02/09/2023 | Tranmere Rovers | - | Wrexham AFC | 0:1 | ||||
| 7. Round | 09/09/2023 | Colchester United | - | Tranmere Rovers | 2:0 |  |
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| 8. Round | 16/09/2023 | Crawley Town | - | Tranmere Rovers | 3:2 | |
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| 9. Round | 23/09/2023 | Tranmere Rovers | - | Accrington Stanley | 2:0 | ||||
| 10. Round | 30/09/2023 | AFC Wimbledon | - | Tranmere Rovers | 4:1 | ||||
| 15. Round | 24/10/2023 | Morecambe FC | - | Tranmere Rovers | 1:0 | ||||
| 16. Round | 28/10/2023 | Stockport County | - | Tranmere Rovers | 2:0 | ||||
| 17. Round | 11/11/2023 | Tranmere Rovers | - | Forest Green Rovers | 3:0 | ||||
| 18. Round | 18/11/2023 | Sutton United | - | Tranmere Rovers | 1:1 | ||||
| 22. Round | 16/12/2023 | Walsall FC | - | Tranmere Rovers | 1:0 | ||||
| 26. Round | 01/01/2024 | Tranmere Rovers | - | Notts County | 4:2 | ||||
| 28. Round | 13/01/2024 | Tranmere Rovers | - | Milton Keynes Dons | 1:2 | ||||
| 29. Round | 27/02/2024 | Swindon Town | - | Tranmere Rovers | 3:1 | ||||
| 38. Round | 12/03/2024 | Tranmere Rovers | - | Mansfield Town | 2:1 | ||||
| 41. Round | 29/03/2024 | Bradford City | - | Tranmere Rovers | 2:0 | |
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| 44. Round | 13/04/2024 | Newport County | - | Tranmere Rovers | 1:2 |
England