| 2. Round | 15/08/2009 | Grimsby Town | - | Crewe Alexandra | 0:4 | ||||
| 3. Round | 18/08/2009 | Grimsby Town | - | Rotherham United | 1:2 | ||||
| 4. Round | 22/08/2009 | Bury FC | - | Grimsby Town | 0:1 | ||||
| 5. Round | 29/08/2009 | Grimsby Town | - | Aldershot Town | 1:2 | ||||
| 6. Round | 05/09/2009 | Port Vale FC | - | Grimsby Town | 4:0 | ||||
| 7. Round | 12/09/2009 | Grimsby Town | - | Hereford United | 1:0 |  |
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| 8. Round | 19/09/2009 | Torquay United | - | Grimsby Town | 0:2 | ||||
| 9. Round | 26/09/2009 | Grimsby Town | - | Darlington FC | 1:1 | ||||
| 11. Round | 03/10/2009 | Barnet FC | - | Grimsby Town | 3:0 |  |
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| 12. Round | 10/10/2009 | Grimsby Town | - | Burton Albion | 1:2 | |
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| 13. Round | 17/10/2009 | Grimsby Town | - | Rochdale AFC | 0:2 | ||||
| 15. Round | 30/10/2009 | Grimsby Town | - | Accrington Stanley | 2:2 | ||||
| 16. Round | 14/11/2009 | Northampton Town | - | Grimsby Town | 0:0 | ||||
| 17. Round | 21/11/2009 | Lincoln City | - | Grimsby Town | 0:0 | ||||
| 20. Round | 05/12/2009 | Grimsby Town | - | Dagenham & Redbridge | 1:1 | ||||
| 21. Round | 12/12/2009 | Shrewsbury Town | - | Grimsby Town | 0:0 | ||||
| 27. Round | 16/01/2010 | Grimsby Town | - | Cheltenham Town | 0:0 | |
England