| 8. Round | 11/12/2000 | Slavia Praha | - | Sigma Olomouc | 2:2 | ||||
| 17. Round | 18/02/2001 | Sigma Olomouc | - | Chmel Blsany | 3:0 | ||||
| 18. Round | 04/03/2001 | Sigma Olomouc | - | FC Stavo Artikel Brno | 3:0 | ||||
| 19. Round | 11/03/2001 | Viktoria Žižkov | - | Sigma Olomouc | 2:2 | ||||
| 20. Round | 18/03/2001 | Sigma Olomouc | - | FK Jablonec 97 | 1:0 | ||||
| 21. Round | 31/03/2001 | Dynamo České Budějovice | - | Sigma Olomouc | 3:1 | ||||
| 22. Round | 03/04/2001 | FC Marila Příbram | - | Sigma Olomouc | 0:0 | ||||
| 23. Round | 06/04/2001 | Sigma Olomouc | - | Slavia Praha | 1:5 | ||||
| 24. Round | 15/04/2001 | FK Drnovice | - | Sigma Olomouc | 1:2 |  |
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| 25. Round | 21/04/2001 | Sigma Olomouc | - | 1. FC Synot Stare Mesto | 3:2 | ||||
| 28. Round | 11/05/2001 | Slovan Liberec | - | Sigma Olomouc | 2:0 | ||||
| 29. Round | 18/05/2001 | Sigma Olomouc | - | Baník Ostrava | 0:0 | ||||
| 30. Round | 25/05/2001 | AC Sparta Praha | - | Sigma Olomouc | 0:1 |
Czech Republic