| 1. Round | 07/08/2009 | Karlsruher SC | - | Alemannia Aachen | 1:1 |  |
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| 2. Round | 14/08/2009 | SC Paderborn 07 | - | Karlsruher SC | 2:0 | |
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| 3. Round | 24/08/2009 | TSV 1860 München | - | Karlsruher SC | 1:3 | |
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| 4. Round | 28/08/2009 | Karlsruher SC | - | FC St. Pauli | 0:4 | ||||
| 5. Round | 14/09/2009 | Energie Cottbus | - | Karlsruher SC | 2:4 | ||||
| 6. Round | 18/09/2009 | Karlsruher SC | - | FSV Frankfurt | 2:0 | ||||
| 8. Round | 05/10/2009 | Karlsruher SC | - | Arminia Bielefeld | 0:1 | ||||
| 9. Round | 18/10/2009 | FC Augsburg | - | Karlsruher SC | 1:1 | |
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| 10. Round | 25/10/2009 | Karlsruher SC | - | Rot-Weiß Oberhausen | 1:1 | |
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| 12. Round | 08/11/2009 | Karlsruher SC | - | 1. FC Union Berlin | 3:2 | ||||
| 13. Round | 21/11/2009 | Hansa Rostock | - | Karlsruher SC | 2:1 | ||||
| 14. Round | 27/11/2009 | Karlsruher SC | - | TuS Koblenz | 2:1 | ||||
| 15. Round | 06/12/2009 | Rot Weiss Ahlen | - | Karlsruher SC | 1:3 | ||||
| 16. Round | 11/12/2009 | Karlsruher SC | - | MSV Duisburg | 0:1 | |
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| 17. Round | 19/12/2009 | SpVgg Greuther Fürth | - | Karlsruher SC | 1:4 | ||||
| 18. Round | 15/01/2010 | Alemannia Aachen | - | Karlsruher SC | 3:1 | ||||
| 19. Round | 24/01/2010 | Karlsruher SC | - | SC Paderborn 07 | 1:2 |
Croatia