| 2. Round | 10/08/2013 | MVV | - | Jong FC Twente | 1:0 | ||||
| 5. Round | 30/08/2013 | Jong Ajax | - | Jong FC Twente | 2:1 | ||||
| 6. Round | 02/09/2013 | Jong FC Twente | - | Almere City FC | 0:0 | ||||
| 8. Round | 16/09/2013 | Jong FC Twente | - | FC Emmen | 1:2 | ||||
| 9. Round | 20/09/2013 | De Graafschap | - | Jong FC Twente | 3:3 | ||||
| 12. Round | 14/10/2013 | Jong FC Twente | - | FC Dordrecht | 2:0 | ||||
| 14. Round | 28/10/2013 | Jong FC Twente | - | VVV-Venlo | 1:1 | ||||
| 16. Round | 08/11/2013 | Jong FC Twente | - | Telstar | 3:0 | ||||
| 19. Round | 30/11/2013 | Jong FC Twente | - | Sparta Rotterdam | 1:0 | ||||
| 21. Round | 16/12/2013 | Jong FC Twente | - | Jong Ajax | 0:1 | ||||
| 25. Round | 31/01/2014 | FC Oss | - | Jong FC Twente | 2:1 | ||||
| 27. Round | 14/02/2014 | VVV-Venlo | - | Jong FC Twente | 3:0 | ||||
| 28. Round | 21/02/2014 | Fortuna Sittard | - | Jong FC Twente | 4:1 | ||||
| 32. Round | 21/03/2014 | FC Dordrecht | - | Jong FC Twente | 3:1 | ||||
| 36. Round | 18/04/2014 | Helmond Sport | - | Jong FC Twente | 5:1 |
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