| 1. Round | 12/09/2020 | Scunthorpe United | - | Newport County | 1:1 | ||||
| 2. Round | 19/09/2020 | Crawley Town | - | Scunthorpe United | 1:0 | ||||
| 11. Round | 17/11/2020 | Port Vale FC | - | Scunthorpe United | 0:1 | ||||
| 13. Round | 21/11/2020 | Scunthorpe United | - | Morecambe FC | 1:1 | ||||
| 14. Round | 24/11/2020 | Scunthorpe United | - | Bolton Wanderers | 0:1 | ||||
| 15. Round | 01/12/2020 | Harrogate Town | - | Scunthorpe United | 2:5 | ||||
| 16. Round | 05/12/2020 | Scunthorpe United | - | Leyton Orient | 2:0 | ||||
| 10. Round | 08/12/2020 | Scunthorpe United | - | Colchester United | 0:1 | ||||
| 24. Round | 16/01/2021 | Barrow AFC | - | Scunthorpe United | 1:0 | ||||
| 31. Round | 23/02/2021 | Bolton Wanderers | - | Scunthorpe United | 2:0 |  |
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| 32. Round | 27/02/2021 | Scunthorpe United | - | Cheltenham Town | 0:2 | ||||
| 33. Round | 02/03/2021 | Cambridge United | - | Scunthorpe United | 0:1 | ||||
| 34. Round | 06/03/2021 | Salford City | - | Scunthorpe United | 1:1 | ||||
| 29. Round | 13/04/2021 | Morecambe FC | - | Scunthorpe United | 4:1 | ||||
| 42. Round | 17/04/2021 | Forest Green Rovers | - | Scunthorpe United | 3:2 |  |
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| 46. Round | 08/05/2021 | Scunthorpe United | - | Stevenage FC | 0:1 |
England