| 3. Round | 26/09/2020 | Cambridge United | - | Tranmere Rovers | 0:0 | ||||
| 5. Round | 10/10/2020 | Cambridge United | - | Newport County | 2:1 | ||||
| 6. Round | 17/10/2020 | Scunthorpe United | - | Cambridge United | 0:5 | ||||
| 7. Round | 20/10/2020 | Cambridge United | - | Port Vale FC | 3:1 | ||||
| 8. Round | 24/10/2020 | Cambridge United | - | Bolton Wanderers | 1:1 | ||||
| 11. Round | 03/11/2020 | Cambridge United | - | Salford City | 2:1 | ||||
| 12. Round | 14/11/2020 | Cambridge United | - | Barrow AFC | 1:1 | ||||
| 17. Round | 12/12/2020 | Forest Green Rovers | - | Cambridge United | 2:0 | ||||
| 18. Round | 15/12/2020 | Cambridge United | - | Colchester United | 2:1 | ||||
| 19. Round | 19/12/2020 | Bradford City | - | Cambridge United | 1:0 | ||||
| 20. Round | 26/12/2020 | Cambridge United | - | Leyton Orient | 2:1 | ||||
| 21. Round | 29/12/2020 | Stevenage FC | - | Cambridge United | 1:0 |  |
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| 22. Round | 02/01/2021 | Grimsby Town | - | Cambridge United | 1:2 | ||||
| 23. Round | 09/01/2021 | Cambridge United | - | Harrogate Town | 2:1 | |
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| 24. Round | 16/01/2021 | Colchester United | - | Cambridge United | 1:1 | |
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| 13. Round | 19/01/2021 | Southend United | - | Cambridge United | 1:2 |
England