| 14. Round | 19/02/2021 | US Biskra | - | USM Alger | 1:0 | ||||
| 15. Round | 26/02/2021 | JS Saoura | - | US Biskra | 4:0 | ||||
| 17. Round | 13/03/2021 | AS Aïn M'lila | - | US Biskra | 2:1 | ||||
| 19. Round | 21/03/2021 | US Biskra | - | CR Bélouizdad | 1:0 | ||||
| 20. Round | 04/05/2021 | JSM Skikda | - | US Biskra | 2:1 | ||||
| 21. Round | 16/05/2021 | NA Hussein Dey | - | US Biskra | 1:1 | ||||
| 22. Round | 22/05/2021 | US Biskra | - | NC Magra | 1:0 | ||||
| 23. Round | 26/05/2021 | Paradou AC | - | US Biskra | 1:1 | ||||
| 24. Round | 30/05/2021 | US Biskra | - | WA Tlemcen | 2:0 | ||||
| 25. Round | 13/06/2021 | JS Kabylie | - | US Biskra | 1:1 | ||||
| 26. Round | 19/06/2021 | US Biskra | - | RC Rélizane | 3:2 | ||||
| 28. Round | 01/07/2021 | US Biskra | - | Olympique Médéa | 2:2 | ||||
| 29. Round | 04/07/2021 | CAB Bou Arreridj | - | US Biskra | 0:0 | ||||
| 30. Round | 08/07/2021 | US Biskra | - | MC Alger | 2:1 | ||||
| 31. Round | 13/07/2021 | CS Constantine | - | US Biskra | 1:1 | ||||
| 32. Round | 17/07/2021 | US Biskra | - | MC Oran | 1:0 | ||||
| 34. Round | 27/07/2021 | US Biskra | - | JS Saoura | 1:2 |  |
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| 36. Round | 16/08/2021 | US Biskra | - | AS Aïn M'lila | 1:0 | ||||
| 37. Round | 21/08/2021 | US Biskra | - | ASO Chlef | 2:0 | ||||
| 38. Round | 24/08/2021 | CR Bélouizdad | - | US Biskra | 4:2 |
Algeria