| 1. Round | 23/03/2022 | New York RB II | - | Louisville City FC | 0:2 | ||||
| 1. Round | 26/03/2022 | Louisville City FC | - | Indy Eleven | 1:1 | ||||
| 2. Round | 17/04/2022 | San Diego Loyal | - | Louisville City FC | 2:5 | ||||
| 2. Round | 23/04/2022 | Charleston Battery | - | Louisville City FC | 1:1 |  |
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| 3. Round | 01/05/2022 | Louisville City FC | - | Pittsburgh Riverhounds | 2:0 | ||||
| 3. Round | 14/05/2022 | Los Angeles Galaxy II | - | Louisville City FC | 4:3 | |
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| 3. Round | 22/05/2022 | Louisville City FC | - | Monterey Bay FC | 0:2 | ||||
| 5. Round | 10/07/2022 | Louisville City FC | - | New York RB II | 6:0 | ||||
| 5. Round | 17/07/2022 | Memphis 901 | - | Louisville City FC | 1:2 | ||||
| 6. Round | 21/08/2022 | Detroit City FC | - | Louisville City FC | 0:1 | ||||
| 7. Round | 01/09/2022 | Indy Eleven | - | Louisville City FC | 2:1 | ||||
| 7. Round | 04/09/2022 | Louisville City FC | - | Sacramento Republic | 3:1 | ||||
| 7. Round | 08/09/2022 | FC Tulsa | - | Louisville City FC | 2:2 | ||||
| 8. Round | 02/10/2022 | Rio Grande Valley FC | - | Louisville City FC | 0:1 | |
Spain