| 2. Round | 17/05/2021 | Rio Grande Valley FC | - | San Antonio FC | 2:1 | ||||
| 2. Round | 23/05/2021 | El Paso Locomotive | - | Rio Grande Valley FC | 1:0 | ||||
| 2. Round | 30/05/2021 | San Antonio FC | - | Rio Grande Valley FC | 1:1 |  |
|||
| 3. Round | 06/06/2021 | Miami FC | - | Rio Grande Valley FC | 2:4 | ||||
| 3. Round | 13/06/2021 | Rio Grande Valley FC | - | Real Monarchs SLC | 1:0 | |
|||
| 3. Round | 17/06/2021 | Rio Grande Valley FC | - | Austin Bold | 1:2 | ||||
| 3. Round | 20/06/2021 | San Antonio FC | - | Rio Grande Valley FC | 1:1 | |
|||
| 4. Round | 08/07/2021 | Austin Bold | - | Rio Grande Valley FC | 1:1 | ||||
| 4. Round | 18/07/2021 | Orange County SC | - | Rio Grande Valley FC | 0:2 | ||||
| 4. Round | 25/07/2021 | Rio Grande Valley FC | - | Austin Bold | 1:1 | ||||
| 5. Round | 05/08/2021 | Real Monarchs SLC | - | Rio Grande Valley FC | 0:3 |  |
|||
| 5. Round | 09/08/2021 | Rio Grande Valley FC | - | San Antonio FC | 1:2 | ||||
| 5. Round | 18/08/2021 | Austin Bold | - | Rio Grande Valley FC | 1:1 | ||||
| 5. Round | 22/08/2021 | El Paso Locomotive | - | Rio Grande Valley FC | 3:1 |
Colombia