| 1. Round | 13/08/2023 | Almere City FC | - | FC Twente | 1:4 | ||||
| 2. Round | 20/08/2023 | FC Twente | - | PEC Zwolle | 3:1 | ||||
| 12. Round | 11/11/2023 | FC Twente | - | NEC Nijmegen | 3:3 | ||||
| 13. Round | 25/11/2023 | FC Twente | - | PSV Eindhoven | 0:3 | ||||
| 15. Round | 08/12/2023 | FC Twente | - | SBV Excelsior | 4:2 | ||||
| 17. Round | 13/01/2024 | FC Twente | - | AZ Alkmaar | 2:1 | ||||
| 18. Round | 20/01/2024 | NEC Nijmegen | - | FC Twente | 1:0 | ||||
| 19. Round | 28/01/2024 | Feyenoord | - | FC Twente | 0:0 | ||||
| 20. Round | 03/02/2024 | FC Twente | - | RKC Waalwijk | 3:0 | ||||
| 21. Round | 10/02/2024 | SBV Excelsior | - | FC Twente | 0:3 |  |
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| 22. Round | 18/02/2024 | FC Twente | - | FC Utrecht | 0:1 | ||||
| 23. Round | 25/02/2024 | FC Twente | - | Go Ahead Eagles | 3:0 |  |
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| 24. Round | 02/03/2024 | Vitesse | - | FC Twente | 1:2 | |
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| 25. Round | 09/03/2024 | FC Twente | - | Sparta Rotterdam | 2:1 | ||||
| 26. Round | 17/03/2024 | PSV Eindhoven | - | FC Twente | 1:0 |
Netherlands