| 1. Round | 12/07/2009 | Real Cartagena | - | Santa Fe | 1:0 | ||||
| 2. Round | 19/07/2009 | Santa Fe | - | Boyacá Chicó | 4:2 |  |
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| 3. Round | 26/07/2009 | Atlético Huila | - | Santa Fe | 2:1 | ||||
| 4. Round | 02/08/2009 | Envigado FC | - | Santa Fe | 2:3 | ||||
| 5. Round | 09/08/2009 | Santa Fe | - | Independiente Medellín | 0:3 dec. | ||||
| 6. Round | 17/08/2009 | Deportivo Pereira | - | Santa Fe | 0:0 | ||||
| 7. Round | 22/08/2009 | Santa Fe | - | La Equidad | 3:0 |  |
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| 8. Round | 30/08/2009 | América de Cali | - | Santa Fe | 1:1 | |
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| 9. Round | 13/09/2009 | Santa Fe | - | Millonarios | 4:2 | |
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| 12. Round | 04/10/2009 | Santa Fe | - | Deportes Tolima | 1:1 | ||||
| 13. Round | 18/10/2009 | Millonarios | - | Santa Fe | 1:1 | |
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| 14. Round | 22/10/2009 | Santa Fe | - | Cúcuta Deportivo | 1:1 | |
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| 15. Round | 25/10/2009 | Atlético Nacional | - | Santa Fe | 1:2 | ||||
| 16. Round | 01/11/2009 | Santa Fe | - | Once Caldas | 2:1 | |
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Chile