| 1. Round | 13/08/2021 | NK Sesvete | - | NK Osijek II | 1:4 |  |
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| 2. Round | 20/08/2021 | NK Osijek II | - | Dinamo Zagreb II | 1:1 | |
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| 3. Round | 28/08/2021 | NK Dugopolje | - | NK Osijek II | 0:0 | ||||
| 4. Round | 10/09/2021 | NK Osijek II | - | NK Solin | 3:2 | ||||
| 5. Round | 15/09/2021 | NK Dubrava | - | NK Osijek II | 3:0 | ||||
| 6. Round | 19/09/2021 | NK Osijek II | - | NK Croatia Zmijavci | 1:2 | ||||
| 7. Round | 26/09/2021 | HNK Orijent Rijeka | - | NK Osijek II | 0:1 | ||||
| 8. Round | 02/10/2021 | NK Osijek II | - | NK Opatija | 0:2 | ||||
| 9. Round | 16/10/2021 | HNK Cibalia | - | NK Osijek II | 1:0 | ||||
| 10. Round | 24/10/2021 | NK Osijek II | - | NK Varaždin | 0:0 |  |
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| 12. Round | 07/11/2021 | NK Osijek II | - | NK Rudeš | 2:1 | ||||
| 11. Round | 12/11/2021 | NK Inter Zaprešić | - | NK Osijek II | 2:0 | |
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| 13. Round | 19/11/2021 | NK Kustošija | - | NK Osijek II | 0:0 | |
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| 15. Round | 05/12/2021 | NK BSK Bijelo Brdo | - | NK Osijek II | 0:2 |
Croatia