| 5. Round | 30/08/2009 | Feyenoord | - | FC Twente | 1:1 | ||||
| 6. Round | 12/09/2009 | FC Twente | - | FC Utrecht | 3:2 | ||||
| 7. Round | 20/09/2009 | sc Heerenveen | - | FC Twente | 0:2 | ||||
| 11. Round | 25/10/2009 | FC Twente | - | FC Groningen | 4:0 | ||||
| 12. Round | 31/10/2009 | Roda JC Kerkrade | - | FC Twente | 1:2 | ||||
| 13. Round | 08/11/2009 | FC Twente | - | AFC Ajax | 1:0 | ||||
| 14. Round | 21/11/2009 | FC Twente | - | Vitesse | 1:0 | ||||
| 15. Round | 28/11/2009 | Willem II | - | FC Twente | 1:3 | ||||
| 16. Round | 06/12/2009 | NEC Nijmegen | - | FC Twente | 3:4 | ||||
| 17. Round | 12/12/2009 | FC Twente | - | NAC Breda | 3:1 |
Iraq