| 2. Round | 17/04/2016 | Columbus Crew | - | New York City FC | 3:2 | ||||
| 2. Round | 24/04/2016 | Columbus Crew | - | Houston Dynamo | 1:0 | ||||
| 2. Round | 30/04/2016 | Seattle Sounders | - | Columbus Crew | 1:0 | ||||
| 3. Round | 08/05/2016 | Columbus Crew | - | Impact de Montréal | 4:4 | ||||
| 3. Round | 21/05/2016 | Toronto FC | - | Columbus Crew | 0:0 | ||||
| 3. Round | 29/05/2016 | Columbus Crew | - | Real Salt Lake | 4:3 | ||||
| 4. Round | 19/06/2016 | Columbus Crew | - | Impact de Montréal | 0:0 | ||||
| 5. Round | 04/07/2016 | Sporting Kansas City | - | Columbus Crew | 3:2 | ||||
| 6. Round | 21/08/2016 | New England Revolution | - | Columbus Crew | 0:2 | ||||
| 7. Round | 18/09/2016 | Orlando City | - | Columbus Crew | 1:4 |
Guatemala