| 1. Round | 03/08/2024 | Hamilton Academical | - | Ayr United | 0:2 | ||||
| 2. Round | 09/08/2024 | Ayr United | - | Airdrieonians FC | 5:0 | ||||
| 11. Round | 17/08/2024 | Ayr United | - | Hamilton Academical | 3:2 | ||||
| 3. Round | 24/08/2024 | Ayr United | - | Raith Rovers | 2:0 | ||||
| 6. Round | 21/09/2024 | Queen's Park FC | - | Ayr United | 1:1 |  |
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| 7. Round | 28/09/2024 | Ayr United | - | Greenock Morton FC | 1:0 | |
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| 8. Round | 05/10/2024 | Falkirk FC | - | Ayr United | 2:0 | ||||
| 10. Round | 26/10/2024 | Airdrieonians FC | - | Ayr United | 0:1 | ||||
| 12. Round | 01/11/2024 | Raith Rovers | - | Ayr United | 2:0 |
England