| 1. Round | 27/07/2019 | TSV Steinbach Haiger | - | 1. FSV Mainz 05 II | 3:0 | ||||
| 2. Round | 04/08/2019 | 1. FSV Mainz 05 II | - | Rot-Weiß Koblenz | 2:0 | ||||
| 3. Round | 10/08/2019 | FSV Frankfurt | - | 1. FSV Mainz 05 II | 1:1 | ||||
| 4. Round | 14/08/2019 | 1. FSV Mainz 05 II | - | TSG Balingen | 1:0 | ||||
| 5. Round | 17/08/2019 | FC-Astoria Walldorf | - | 1. FSV Mainz 05 II | 0:0 | ||||
| 6. Round | 25/08/2019 | 1. FSV Mainz 05 II | - | 1899 Hoffenheim II | 0:2 | ||||
| 9. Round | 15/09/2019 | 1. FSV Mainz 05 II | - | FC Gießen | 1:3 | ||||
| 10. Round | 22/09/2019 | SSV Ulm 1846 | - | 1. FSV Mainz 05 II | 0:1 |  |
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| 11. Round | 29/09/2019 | 1. FSV Mainz 05 II | - | SC Freiburg II | 3:2 | ||||
| 12. Round | 05/10/2019 | VfR Aalen | - | 1. FSV Mainz 05 II | 0:0 | ||||
| 16. Round | 02/11/2019 | FC 08 Homburg | - | 1. FSV Mainz 05 II | 2:1 |  |
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| 19. Round | 01/12/2019 | Rot-Weiß Koblenz | - | 1. FSV Mainz 05 II | 1:4 | ||||
| 21. Round | 21/02/2020 | TSG Balingen | - | 1. FSV Mainz 05 II | 1:5 | |
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| 22. Round | 01/03/2020 | 1. FSV Mainz 05 II | - | FC-Astoria Walldorf | 0:1 | ||||
| 23. Round | 07/03/2020 | 1899 Hoffenheim II | - | 1. FSV Mainz 05 II | 1:4 | |
Belgium