| 1. Round | 05/08/2023 | Wrexham AFC | - | Milton Keynes Dons | 3:5 | ||||
| 2. Round | 12/08/2023 | Milton Keynes Dons | - | Tranmere Rovers | 1:0 | ||||
| 3. Round | 15/08/2023 | Crawley Town | - | Milton Keynes Dons | 2:1 | ||||
| 4. Round | 19/08/2023 | Colchester United | - | Milton Keynes Dons | 2:3 | ||||
| 5. Round | 26/08/2023 | Milton Keynes Dons | - | Doncaster Rovers | 2:1 |  |
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| 6. Round | 02/09/2023 | Crewe Alexandra | - | Milton Keynes Dons | 3:1 | ||||
| 7. Round | 09/09/2023 | Milton Keynes Dons | - | Notts County | 1:1 | |
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| 8. Round | 16/09/2023 | Milton Keynes Dons | - | Stockport County | 1:2 | |
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| 13. Round | 14/10/2023 | Milton Keynes Dons | - | Barrow AFC | 2:2 | ||||
| 14. Round | 21/10/2023 | Accrington Stanley | - | Milton Keynes Dons | 1:0 |
England