| 1. Round | 31/07/2022 | Slavia Praha B | - | 1. SK Prostějov | 0:1 |  |
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| 2. Round | 06/08/2022 | 1. SK Prostějov | - | FK Třinec | 1:1 | ||||
| 3. Round | 14/08/2022 | FK Varnsdorf | - | 1. SK Prostějov | 2:0 | ||||
| 4. Round | 20/08/2022 | 1. SK Prostějov | - | Sigma Olomouc B | 1:3 | ||||
| 6. Round | 31/08/2022 | 1. SK Prostějov | - | MFK Chrudim | 1:1 | ||||
| 7. Round | 04/09/2022 | FC Sellier & Bellot Vlašim | - | 1. SK Prostějov | 6:1 | ||||
| 5. Round | 07/09/2022 | FK Viagem Příbram | - | 1. SK Prostějov | 2:2 | |
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| 8. Round | 10/09/2022 | 1. SK Prostějov | - | FK MAS Táborsko | 5:0 | |
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| 9. Round | 17/09/2022 | MFK Vyškov | - | 1. SK Prostějov | 4:1 | ||||
| 10. Round | 01/10/2022 | 1. SK Prostějov | - | AC Sparta Praha B | 0:2 | ||||
| 13. Round | 22/10/2022 | Sk Lisen | - | 1. SK Prostějov | 2:3 | ||||
| 15. Round | 05/11/2022 | 1. SK Prostějov | - | FK Dukla Praha | 4:2 | ||||
| 16. Round | 12/11/2022 | FK Třinec | - | 1. SK Prostějov | 2:2 |
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