| 4. Round | 03/09/2023 | FC Volendam | - | FC Twente | 0:2 | ||||
| 5. Round | 17/09/2023 | FC Twente | - | AFC Ajax | 3:1 | ||||
| 7. Round | 30/09/2023 | FC Twente | - | sc Heerenveen | 1:0 | ||||
| 8. Round | 08/10/2023 | Fortuna Sittard | - | FC Twente | 0:3 | ||||
| 10. Round | 29/10/2023 | FC Twente | - | Feyenoord | 2:1 | ||||
| 11. Round | 05/11/2023 | FC Utrecht | - | FC Twente | 1:1 | ||||
| 13. Round | 25/11/2023 | FC Twente | - | PSV Eindhoven | 0:3 | ||||
| 14. Round | 03/12/2023 | Go Ahead Eagles | - | FC Twente | 1:3 | ||||
| 15. Round | 08/12/2023 | FC Twente | - | SBV Excelsior | 4:2 | ||||
| 16. Round | 17/12/2023 | Sparta Rotterdam | - | FC Twente | 2:2 | ||||
| 18. Round | 20/01/2024 | NEC Nijmegen | - | FC Twente | 1:0 |  |
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| 19. Round | 28/01/2024 | Feyenoord | - | FC Twente | 0:0 | ||||
| 24. Round | 02/03/2024 | Vitesse | - | FC Twente | 1:2 | ||||
| 27. Round | 31/03/2024 | FC Twente | - | Heracles Almelo | 1:0 | ||||
| 32. Round | 05/05/2024 | AZ Alkmaar | - | FC Twente | 2:1 | ||||
| 34. Round | 19/05/2024 | PEC Zwolle | - | FC Twente | 1:2 |
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