| 1. Round | 10/08/2024 | NEC Nijmegen | - | FC Twente | 1:2 |  |
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| 2. Round | 17/08/2024 | FC Twente | - | Sparta Rotterdam | 1:1 | ||||
| 6. Round | 22/09/2024 | Almere City FC | - | FC Twente | 0:5 | ||||
| 7. Round | 29/09/2024 | FC Twente | - | NAC Breda | 1:0 | ||||
| 9. Round | 19/10/2024 | RKC Waalwijk | - | FC Twente | 2:2 | ||||
| 10. Round | 27/10/2024 | FC Twente | - | Heracles Almelo | 5:0 | ||||
| 14. Round | 01/12/2024 | FC Twente | - | Go Ahead Eagles | 3:2 | ||||
| 16. Round | 15/12/2024 | FC Twente | - | FC Groningen | 2:0 | ||||
| 17. Round | 21/12/2024 | AZ Alkmaar | - | FC Twente | 1:0 | ||||
| 18. Round | 12/01/2025 | FC Twente | - | Willem II | 6:2 | ||||
| 19. Round | 19/01/2025 | NAC Breda | - | FC Twente | 2:1 | ||||
| 22. Round | 09/02/2025 | sc Heerenveen | - | FC Twente | 3:3 | ||||
| 26. Round | 16/03/2025 | FC Twente | - | Feyenoord | 2:6 | ||||
| 27. Round | 30/03/2025 | Heracles Almelo | - | FC Twente | 2:1 | ||||
| 29. Round | 13/04/2025 | PEC Zwolle | - | FC Twente | 1:1 | |
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| 30. Round | 24/04/2025 | FC Twente | - | PSV Eindhoven | 1:3 | ||||
| 31. Round | 04/05/2025 | Sparta Rotterdam | - | FC Twente | 0:2 | ||||
| 32. Round | 11/05/2025 | FC Twente | - | FC Utrecht | 2:0 | ||||
| 33. Round | 14/05/2025 | FC Twente | - | AZ Alkmaar | 2:3 |  |
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| 34. Round | 18/05/2025 | AFC Ajax | - | FC Twente | 2:0 |
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