| 14. Round | 13/01/2001 | PAS Giannina | - | OFI Heraklion | 1:2 | ||||
| 16. Round | 26/01/2001 | Aris Saloniki | - | OFI Heraklion | 2:0 |  |
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| 17. Round | 02/02/2001 | Olympiakos Piraeus | - | OFI Heraklion | 5:0 | ||||
| 18. Round | 10/02/2001 | OFI Heraklion | - | Paniliakos Pyrgos | 2:2 | ||||
| 19. Round | 16/02/2001 | Panathinaikos | - | OFI Heraklion | 2:0 | ||||
| 20. Round | 24/02/2001 | OFI Heraklion | - | PAOK Saloniki | 0:0 | ||||
| 21. Round | 03/03/2001 | Skoda Xanthi | - | OFI Heraklion | 3:2 | ||||
| 23. Round | 17/03/2001 | Athinaikos | - | OFI Heraklion | 2:3 | ||||
| 24. Round | 31/03/2001 | OFI Heraklion | - | Ethnikos Asteras | 2:2 | ||||
| 25. Round | 07/04/2001 | OFI Heraklion | - | Ionikos Nikea | 3:1 | ||||
| 27. Round | 28/04/2001 | OFI Heraklion | - | Panachaiki GE | 0:1 | ||||
| 28. Round | 05/05/2001 | PS Kalamata | - | OFI Heraklion | 1:4 | |
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| 30. Round | 26/05/2001 | Panionios GSS | - | OFI Heraklion | 0:2 |
Greece