| 1. Round | 09/03/2025 | Charleston Battery | - | Louisville City FC | 1:2 | ||||
| 2. Round | 16/03/2025 | Birmingham Legion | - | Louisville City FC | 1:1 | ||||
| 3. Round | 22/03/2025 | Louisville City FC | - | Loudoun United | 2:0 | ||||
| 4. Round | 29/03/2025 | Louisville City FC | - | Detroit City FC | 2:0 | ||||
| 5. Round | 06/04/2025 | Sacramento Republic | - | Louisville City FC | 1:1 |  |
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| 7. Round | 20/04/2025 | Tampa Bay Rowdies | - | Louisville City FC | 1:2 | ||||
| 8. Round | 02/05/2025 | Louisville City FC | - | Lexington SC | 2:0 | ||||
| 9. Round | 11/05/2025 | Louisville City FC | - | Pittsburgh Riverhounds | 0:0 | ||||
| 10. Round | 18/05/2025 | Hartford Athletic | - | Louisville City FC | 1:1 | ||||
| 11. Round | 25/05/2025 | Monterey Bay FC | - | Louisville City FC | 0:2 |  |
France