| 1. Round | 09/03/2025 | Miami FC | - | Detroit City FC | 0:2 | ||||
| 2. Round | 15/03/2025 | Colorado Springs Switchbacks | - | Detroit City FC | 1:2 | ||||
| 3. Round | 22/03/2025 | Detroit City FC | - | Birmingham Legion | 2:2 |  |
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| 4. Round | 29/03/2025 | Louisville City FC | - | Detroit City FC | 2:0 | ||||
| 5. Round | 05/04/2025 | Detroit City FC | - | Monterey Bay FC | 0:0 | ||||
| 6. Round | 13/04/2025 | Phoenix Rising | - | Detroit City FC | 2:3 | ||||
| 7. Round | 19/04/2025 | Detroit City FC | - | Rhode Island FC | 2:0 | ||||
| 9. Round | 11/05/2025 | Hartford Athletic | - | Detroit City FC | 0:0 | ||||
| 10. Round | 18/05/2025 | Birmingham Legion | - | Detroit City FC | 1:1 | ||||
| 11. Round | 24/05/2025 | Detroit City FC | - | Charleston Battery | 1:3 |
England