| 1. Round | 14/08/2016 | Jong FC Twente | - | Amsterdamsche FC | 1:2 | ||||
| 16. Round | 03/12/2016 | FC Lienden | - | Jong FC Twente | 1:1 | ||||
| 17. Round | 10/12/2016 | Jong FC Twente | - | Koninklijke HFC | 1:1 | ||||
| 18. Round | 18/12/2016 | Amsterdamsche FC | - | Jong FC Twente | 4:0 | ||||
| 19. Round | 16/01/2017 | Jong FC Twente | - | Vitesse (J) | 1:5 | ||||
| 21. Round | 28/01/2017 | Jong FC Twente | - | GVVV | 3:1 | ||||
| 22. Round | 04/02/2017 | BVV Barendrecht | - | Jong FC Twente | 1:1 | ||||
| 23. Round | 11/02/2017 | Jong FC Twente | - | VV Katwijk | 0:1 |  |
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| 24. Round | 20/02/2017 | AZ Alkmaar (J) | - | Jong FC Twente | 1:0 | ||||
| 26. Round | 11/03/2017 | Jong FC Twente | - | SV Spakenburg | 1:1 | ||||
| 27. Round | 19/03/2017 | VV Sint Bavo | - | Jong FC Twente | 2:1 | ||||
| 28. Round | 27/03/2017 | Jong FC Twente | - | Sparta Rotterdam (J) | 0:3 |
Netherlands